Saoirse Ronan (Mary Stuart), Margot Robbie (Queen Elizabeth I), Jack Lowden (Henry Darnley), Joe Alwyn (Robert Dudley), David Tennant (John Knox), Guy Pearce (William Cecil), Gemma Chan (Bess of Hardwick) more..., Martin Compston (Earl of Bothwell), Ismael Cruz Córdova (David Rizzio), Brendan Coyle (Earl of Lennox), Ian Hart (Lord Maitland), James McArdle (James, Earl of Moray), Richard Cant (Thomas Andrews), Guy Rhys (Earl of Kent), Thom Petty (Earl of Shrewsbury), Izuka Hoyle (Mary Seton), John Ramm (Bull), Simon Russell Beale (Robert Beale), Maria Dragus (Mary Fleming), Liah O'Prey (Mary Livingston), Eileen O'Higgins (Mary Beaton), Greg Miller Burns (Thomas Hepburn), Aneurin Pascoe (John Hepburn), Adrian Derrick-Palmer (George Dalgleish), Kal Sabir (The Laird of Ramorny), Luke Kidd (James Haliburton), Adrian Lester (Lord Randolph), Georgia Burnell (Kate Carey), Alan Turkington (Thomas Jenye), Euan MacNaughton (William Kirkcaldy of Grange), Scot Greenan (Hector Maclean), Karen Dunbar (Commoner), Ian Hallard (Sir Richard Knightley), Andrew Rothney (King James I), Angela Bain (Snuffer Woman), Abby Cassidy (Fotheringhay Gentlewoman), Shalisha James-Davis (Fotheringhay Gentlewoman), Adam Bond (Sir William Douglas), Katharine O'Donnelly (Young Holyrood Servant), Grace Molony (Dorothy Stafford) and others.

John Mathieson (Director of Photography), Beau Willimon (Screenplay), Chris Dickens (Editor), James Merifield (Production Design), Alexandra Byrne (Costume Design), John Guy (Book), Max Richter (Original Music Composer) more..., Tim Bevan (Producer), Eric Fellner (Producer), Debra Hayward (Producer), Jane Robertson (Co-Producer), Gina Cromwell (Set Decoration), Richard May (Production Manager), Alex Oakley (First Assistant Director), Ian Wilson (Supervising Sound Editor), Alastair Sirkett (Sound Mixer), Mark Flanagan (Stunts), Jenny Shircore (Makeup Designer), Marc Pilcher (Hairstylist), Jessica Brooks (Makeup Artist), Peter Dillon (Stunts), Kirsten Errington (Music Consultant),