Cate Blanchett (Elizabeth I, Queen of England), Laurence Fox (Sir Christopher Hatton), Clive Owen (Sir Walter Raleigh), Geoffrey Rush (Sir Francis Walsingham), Tom Hollander (Amyas Paulet), Abbie Cornish (Elizabeth Throckmorton) more..., Rhys Ifans (Robert Reston), Samantha Morton (Mary, Queen of Scots), Jordi Mollà (King Philip II of Spain), Eddie Redmayne (Anthony Babington), Adrian Scarborough (Calley), Adam Godley (William Walsingham), Robert Cambrinus (Count Georg von Helfenstein), Christian Brassington (Archduke Charles), David Threlfall (Dr. John Dee), Vidal Sancho (Spanish Minister), John Shrapnel (Lord Howard), Sam Spruell (Torturer), Kelly Hunter (Ursula Walsingham), David Sterne (Cellarman), David Robb (Admiral Sir William Winter), Jonathan Bailey (Courtier), Steve Lately (Walsingham's Servant), Kate Fleetwood (Woman with Baby), Aimee King (Infanta Isabel of Spain), Susan Lynch (Annette), Kristin Coulter Smith (Mary Walsingham), Hayley Burroughs (Queen Elizabeth's Waiting Lady #1), Kirsty McKay (Queen Elizabeth's Waiting Lady #2), Lucia Ruck Keene (Queen Elizabeth's Waiting Lady #3), William Houston (Don Gerau De Spes), Lucienne Venisse-Back (Queen Elizabeth's Waiting Lady #4), Elise McCave (Laundry Woman), Penelope McGhie (Margaret), Coral Beed (First Court Lady), Rosalind Halstead (Second Court Lady), Steven Loton (Manteo), Martin Baron (Wanchese), David Armand (Walsingham's Agent), Steven Robertson (Sir Francis Throckmorton) and others.

Remi Adefarasin (Director of Photography), William Nicholson (Screenplay), Michael Hirst (Screenplay), Jill Bilcock (Editor), Andrew Haddock (Editor), Fiona Weir (Casting), Richard Roberts (Set Decoration) more..., Alexandra Byrne (Costume Design), Craig Armstrong (Original Music Composer), A.R. Rahman (Original Music Composer), David Allday (Art Direction), Christian Huband (Art Direction), Jason Knox-Johnston (Art Direction), Phil Sims (Art Direction), Andy Thomson (Art Direction), Morag Ross (Hairstylist), Dorka Nieradzik (Hairstylist), Marion McCormack (Hairstylist), Susan Howard (Makeup Artist), Joe Hopker (Makeup Artist), Uxue Laguardia (Makeup Artist), Mark Mostyn (Production Manager), Anna Skrein (Art Department Coordinator), James Gemmill (Scenic Artist), Rohan Harris (Scenic Artist), Steve Mitchell (Scenic Artist), Helen Xenopoulos (Assistant Art Director), Ian Whiteford (Greensman), Duncan McDevitt (Sculptor), Stephen McClure (Sculptor), Alexandra Harwood (Sculptor), Steve Deahl (Sculptor), Mark Auguste (Supervising Sound Editor), Tim Cavagin (Sound Re-Recording Mixer), Steve Single (Sound Re-Recording Mixer), Ian Ward (CG Supervisor), Michelle Corney (Visual Effects Producer), Victoria Mowlam (Visual Effects Producer), Aled Robinson (Visual Effects Editor), John Lockwood (Visual Effects Supervisor) and others.