Tom Holland (Peter Parker / Spider-Man), Samuel L. Jackson (Nick Fury), Jake Gyllenhaal (Quentin Beck / Mysterio), Marisa Tomei (May Parker), Jon Favreau (Harold 'Happy' Hogan), Zendaya (Michelle 'MJ' Jones), Jacob Batalon (Ned Leeds) more..., Tony Revolori (Eugene 'Flash' Thompson), Angourie Rice (Betty Brant), Remy Hii (Brad Davis), Martin Starr (Mr. Harrington), J.B. Smoove (Mr. Dell), Jorge Lendeborg Jr. (Jason Ionello), Cobie Smulders (Maria Hill), Numan Acar (Dimitri), Zach Barack (Zach), Zoha Rahman (Zoha), Yasmin Mwanza (Yasmin), Joshua Sinclair-Evans (Josh Spinelli), Tyler Luke Cunningham (Tyler), Sebastián Viveros (Sebastian), Toni Garrn (The Seamstress), Peter Billingsley (William Ginter Riva), Clare Dunne (Victoria), Nicholas Gleaves (Guterman), Claire Rushbrook (Janice), J.K. Simmons (J. Jonah Jameson), Dawn Michelle King (E.D.I.T.H. (voice)), Jeroen van Koningsbrugge (Dutch Soccer Hooligan 1), Michael de Roos (Dutch Soccer Hooligan 2), Jan-Paul Buijs (Dutch Soccer Hooligan 3), Sergio Pierattini (Venetian Glassblower), Anjana Vasan (Queens Reporter), Brian Law (Queens Reporter), Evelyn Mok (Queens Reporter), Tatiana Lunardon (Italian TV Newsreader), Giada Benedetti (Italian Customs Office), Lukáš Bech (Helpful Dutch Man), Alessandro Giuggioli (Italian Street Vendor), Petr Opava (Opera Singer) and others.

Chris McKenna (Writer), Erik Sommers (Screenplay), Stan Lee (Comic Book), Steve Ditko (Comic Book), Michael Giacchino (Music), Leigh Folsom Boyd (Editor), Dan Lebental (Editor) more..., Kevin Feige (Producer), Amy Pascal (Producer), Matt Tolmach (Executive Producer), Thomas M. Hammel (Executive Producer), Louis D'Esposito (Executive Producer), Eric Hauserman Carroll (Executive Producer), Avi Arad (Executive Producer), Victoria Alonso (Executive Producer), Stan Lee (Executive Producer), David Minkowski (Co-Producer), Matthew Stillman (Co-Producer), Claude Paré (Production Design), Tina Jones (Set Decoration), Anna B. Sheppard (Costume Design), Charley Beal (Art Direction), Livia Borgognoni (Art Direction), Nigel Evans (Art Direction), Joe Howard (Art Direction), Gary Jopling (Art Direction), Matthew Kerly (Art Direction), James Lewis (Art Direction), Jiri Matura (Art Direction), Renate Nicolaisen (Art Direction), Grant Armstrong (Supervising Art Director), Jann K. Engel (Supervising Art Director), Donald Mowat (Makeup Artist), Vasilios Tanis (Makeup Artist), Rachael Speke (Makeup Artist), Adrian Rigby (Makeup Artist), Nicola Pope (Makeup Artist), Madlen Mierzwiak (Makeup & Hair), Daniel McGraw (Makeup Artist), Rachel Lennon (Makeup Artist) and others.